The Toca-1-N-WASP complex links filopodia formation to endocytosis.
J Biol Chem. 2009 Feb 11;
Authors: Bu W, Chou AM, Lim KB, Sudhaharan T, Ahmed S
The Transducer of cdc42-dependent actin assembly (Toca-1)-N-WASP complex was isolated as an essential cofactor for Cdc42 driven actin polymerization in vitro. Toca-1 consists of an N-terminal F-BAR domain, followed by a Cdc42 binding site (HR1 domain) and an SH3 domain, (the N-WASP interacting site). N-WASP is an activator of actin nucleation through the Arp2/3 complex. The aim of the present study was to investigate the cellular function of the Toca-1-N-WASP complex. We report that Toca-1 induces filopodia and neurites as does N-WASP in N1E115 neuroblastoma cells. Toca-1 requires the F-BAR domain, Cdc42 binding site and SH3 domain to induce filopodia. Toca-1 and N-WASP both require each other to induce filopodia. The expression of Toca-1 and N-WASP affects the distribution, size and number of Rab5 positive membranes. Toca-1 interacts directly with N-WASP in filopodia and Rab5 membrane as seen by Forster Resonance Energy Transfer (FRET). Thus the Toca-1-N-WASP complex localizes to and induces the formation of filopodia and endocytic vesicles. Lastly, three inhibitors of endocytosis, Dynamin-K44A, Eps1595/295, and clathrin heavy chain RNAi, block Toca-1 induced filopodia formation. Taken together, these data suggest that the Toca-1-N-WASP complex can link filopodia formation to endocytosis.
PMID: 19213734 [PubMed - as supplied by publisher]