Impaired Cholesterol Biosynthesis in a Measles Virus Persistently Infected Neuronal Cell Line.
J Virol. 2009 Mar 18;
Authors: Robinzon S, Dafa-Berger A, Dyer MD, Paeper B, Proll SC, Teal TH, Rom S, Fishman D, Rager-Zisman B, Katze MG
Measles virus remains a substantial cause of morbidity and mortality, producing acute infection with potential for development of viral persistence. To study the events underlying acute and persistent measles virus infection, we performed a global transcriptional analysis on murine neuroblastoma cells that were acutely or persistently infected with measles virus. In general, we found that acute infection induced significantly more gene expression changes than did persistent infection. A functional enrichment analysis to identify which host pathways were perturbed during each of these infections identified several pathways related to cholesterol biosynthesis, including cholesterol metabolic processes, hydroxymethylglutaryl-CoA reductase activity, and acetyl-CoA C-acetyltransferase activity. We also found that measles virus co-localized to lipid rafts in both acute and persistent infection models and that the majority of genes associated with cholesterol synthesis were down-regulated in persistent infection relative to acute infection, suggesting a possible link with the defective viral budding in persistent infection. Further, we found that pharmacological inhibition of cholesterol synthesis resulted in the inhibition of viral budding during acute infection. In summary, persistent measles viral infection was associated with decreased cholesterol synthesis, a lower abundance of cholesterol and lipid rafts in the cell membrane, and inhibition of giant-cell formation and release of viral progeny.
PMID: 19297498 [PubMed - as supplied by publisher]